Table 1. The primer sequences for RT-qPCR.
Primer | Sequence |
---|---|
miR-182 | F: 5′-GGCAATGGTAGAACTCACACT-3′ |
R: 5′-AACATGTACAGTCCATGGATG-3′ | |
HOXA9 | F: 5′-TGCCCTCCAGCCGGCCTTAT-3′ |
R: 5′-CCGCTTTGTGCGGGGATGGT-3 | |
Wnt | F: 5′-GCTAGCGAAAGTCATTGGC -3′ |
R: 5′-CATTGCATCGAAGTCGTG-3′ | |
CTNNB1 | F: 5′-ACAAACTGTTTTGAAAATCCA-3′ |
R: 5′-CGAGTCATTGCATACTGTCC-3′ | |
WIF-1 | F: 5′-CCGAAATGGAGGCTTTTGTA-3′ |
R: 5′-TGGTTGAGCAGTTTGCTTTG-3′ | |
Survivin | F: 5′-CCGAGGGACTGTGTGGGGGTC-3′ |
R: 5′-CCAGGCACAGACCAAGGACGC-3′ | |
CCND1 | F: 5′-AATGGGCCTTAGAATGACTGC-3′ |
R: 5′-ATGCCAACACAGATGGCTTT-3′ | |
C-myc | F: 5′-CTACCAGCAGCAGCAGCAGAGC-3′ |
R: 5′-CGTCCGGGTCGCACATGAA-3′ | |
BIM | F: 5′-CGTAGCGCATGCGATA-3′ |
R: 5′-CATGCCGTATAAGCTAGTT-3′ | |
Bax | F: 5′-GGCCCACCAGCTCTGAGCAGA-3′ |
R: 5′-GCCACGTGGGGGTCCCAAAGT-3′ | |
Mcl-1 | F: 5′-AGAAAGCTGCATCGAACCATTA-3′ |
R: 5′-GGAAGAACAACTCCACAAACCCATC-3′ | |
Bcl-xl | F: 5′-TTGGACAATGGACTGGTTGA-3′ |
R: 5′-GTAGAGTGGATGGTCAGTG-3′ | |
Snail | F: 5′-CTCCTCTACTTCAGCCTCTT-3′ |
R: 5′-CTTCATCAACGTCCTGTGGG-3′ | |
GAPDH | F: 5′-CCACCCATGGCAAATTCCATGGCA-3′ |
R: 5′-TCTAGACGGCAGGTCAGGTCCAC-3′ | |
U6 | F: 5′-CTCGGCTTCGGCAGCACA-3′ |
R: 5′-AACGCTTCACGAATTTGCGT-3′ |
Note: miR-182, microRNA-182; HOXA9, Homeobox A9; WIF-1, Wnt inhibitory factor-1; BIM, B-cell CLL/lymphoma-2 interacting mediator of cell death; Bcl, B-cell CLL/lymphoma; Mcl, Mantle cell lymphoma; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; R, reverse; F, forward; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase.