Figure 2. Xist regulates OS cell proliferation in vitro.

(A) The results of qPCR determined that the relative expression level of Xist by transfection with si-Xist or Xist vector in OS cells, compared with the transfection with control vector. (B) Representative photographs and quantitative analysis of plate colony formation of OS cells transfected with si-Xist, Xist vector or control vector. (C) CCK-8 assays indicated that decreased Xist expression inhibited HOS, MG-63 cells proliferation and increased Xist expression promoted U2OS cells proliferation. Data are represented as mean±SD of three independent experiments. ^*P< 0.05; ^**P < 0.01 compared with control group.