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. 2017 Sep 8;8(60):101406–101417. doi: 10.18632/oncotarget.20738

Figure 7. Xist epigenetically regulated the expression of P21 and thus mediated OS cell proliferation.

Figure 7

(A) The expression of EZH2 in HOS and MG-63 cells were determined by qRT-PCR and western blot after cells transfected with si-EZH2 or si-NC. (B) The expression of P21 in HOS and MG-63 cells were determined by qRT-PCR and western blot after cells transfected with si-EZH2 or si-Xist. (C) ChIP-qPCR of EZH2 and H3K27me3 of the promoter region of P21 after siRNA treatment targeting Xist in MG-63 cells. IgG antibody was used as a negative control. Data are represented as mean±SD of three independent experiments. **P < 0.01 compared with control group.