Table 6. Primer used in this study for qRT-PCR.
Primers | Sequences(5′-3′) | Fragment Size(bp) | Tm (°C) |
---|---|---|---|
InsR | ACCTGTACCCTGGAGAGGTGCCCTCAATGACCGAGCAG | 91 | 60 |
AKT | GTGGCAAGATGTGTATGAGACTGGCTGAGTAGGAGAACTG | 195 | 58 |
PKCα | TGGCGTCCTGTTGTATGAAATCGTCTTCATCTTCACCATCA | 245 | 58 |
PKA | TGTCAACTTCCCGTTCCTGGGCTCACTGAACCTCCCAATCC | 131 | 60 |
PI3K | TGCTACCTTATGCCTGTCTATCCATGTTCTTGTCCTTGAGCCACTG | 159 | 56 |
DPP-IV | TCAGAACTATTCCGTGATGGCGGCCTGTGGCACTCGTTTCAATA | 100 | 60 |
β-actin | AGGGAAATCGTGCGTGACATGAACCGCTCATTGCCGATAG | 150 | 58 |