Figure 1. Isolation of breast CD44⁺CD24^- CSCs and pancreatic CD44⁺CD24⁺ESA⁺ CSCs from MCF7 and L3.6pl cells by flow cytometry.

(A) Typical proportion of CD44⁺CD24^– cells in MCF7 cells. The isolated CD44⁺CD24^– CSCs were obtained as shown in the frame of A and cultured 1d for re-analysis by flow cytometry (Supplementary Figure 1A). (B) Fluorescence activated cell-sorting (FACS) analysis to measure CD44 and CD24 expression of L3.6pl cells (Left) and the patterns of ESA staining of CD24⁺CD44⁺ cells as shown in the frame of panel B Left (Right).