Figure 4. Effect of CCDC69 deletion on expression of p53, acetyl-p53-382 and Bax in response to cisplatin.

(A) The extracted protein samples were analyzed using antibodies against the indicated proteins. Equal protein loading was confirmed by Western blotting for GAPDH. (B) After treatment A2780cis wildtype and A2780cis CCDC69-/- #26 cells with 10 μM cisplatin for 24 h, cytosolic and mitochondrial fractions were obtained. The amounts of protein loaded were 20 μg for cytosol and crude mitochondria. HSP60 and β-actin were used as specific marker proteins for mitochondria and cytosol, respectively.