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. 2017 Sep 28;8(60):101634–101648. doi: 10.18632/oncotarget.21356

Figure 6. DNA methylation analysis of promoter regions of CCDC69 in ovarian cancer cell lines.

Figure 6

(A) Representative methylation patterns in promoter regions of CCDC69 in A2780, A2780cis and SKOV3 ovarian cancer cell lines before treatment with 5-aza-dC. (B) Representative methylation patterns in CCDC69 CpG islands observed in A2780 and A2780cis after treatment with 5-aza-dC. (C) Effect of 5-aza-dC and TSA on CCDC69 expression in A2780, A2780cis and SKOV3 ovarian cancer cell lines. Bisulfite sequencing was performed and the results were visualized by MethVisual [28]. Lollipop displays for each 10 cloning methylation profiles in the genomic context are shown. Filled dots refer to methylated sites, empty dots to non-methylated sites. Each clone of the experiment is displayed separately according to its methylation status. Lengths of the connecting lines correspond to relative genomic distance between CpG sites. Mann–Whitney U-test, (*) P < 0.05; (**) P < 0.01.