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. 2017 Sep 30;8(60):101649–101658. doi: 10.18632/oncotarget.21417

Figure 4. NOB1 is the miR-363target in SKOV3ovarian cancer cells.

Figure 4

(A) Sequence alignment of putative wild-type (WT) and mutant (MT) miR-363-binding sites in the 3’-UTR of NOB1. (B) Dual luciferase reporter assay showing decreased luciferase activity in SKOV3 cells overexpressing miR-363 in combination with wild type NOB1-3’UTR than in SKOV3 cells overexpressing miR-363 in combination with mutant NOB1-3’UTR. (C) qRT-PCR analysis of NOB1 mRNA levels in control and miR-363 overexpressing SKOV3 cells. (D) Western blot analysis of NOB1 protein levels in control and miR-363 overexpressing SKOV3 cells. Note: * denotes p<0.05;** denotes p<0.01.