Skip to main content
. 2017 Sep 30;8(60):101649–101658. doi: 10.18632/oncotarget.21417

Figure 5. NOB1 expression regulates OS progression.

Figure 5

(A) qRT-PCR analysis of NOB1 mRNA levels in SKOV3 cells co-transfected with NOB1 plasmid without 3’UTR in combination with either miR-363 mimic or miR-NC. GAPDH was used as an internal control. (B) Western blot analysis of NOB1 protein levels in SKOV3 cells co-transfected with NOB1 plasmid without 3’UTR in combination with either miR-363 mimic or miR-NC. (C) CCK8 assay showing cell proliferation in SKOV3 cells co-transfected with NOB1 plasmid without 3’UTR in combination with either miR-363 mimic or miR-NC. (D) Estimation of number of colonies in SKOV3 cells stable expressing miR-363 or miR-NC transfected with NOB1 plasmid without 3’UTR. (E) Wound healing assay estimating migration of SKOV3 cells co-transfected with NOB1 plasmid without 3’UTR in combination with either miR-363 mimic or miR-NC. (F) Transwell assay estimating invasiveness of SKOV3 cells co-transfected with NOB1 plasmid without 3’UTR in combination with either miR-363 mimic or miR-NC. Note:* denotes p<0.05;** denotes p<0.01.