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(A) NIH/3T3 fibroblasts were treated for 24h, 48h, 72h, and 96h, respectively. Cell Counting Kit-8 (cck8) assay showed that cell viability was repressed by metformin treatment. (B) Metformin induced a significantly increased apoptosis assessed by flow cytometry in fibroblasts. The histogram showed the percentage of apoptotic cells. Data are expressed as means ± SEM. * P < 0.05; ** P < 0.01.
