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. 2017 Oct 19;8(60):101876–101886. doi: 10.18632/oncotarget.22056

Figure 2. High level of RNA187 promote HCC cells invasion and metastasis both in vitro and in vivo.

Figure 2

(A) qRT-PCR showed RNF187 mRNA in HCCLM3, MHCC97-H, Huh7, PLC, SMMC-7721 and HepG2 cell lines; (B) Western blotting showed RNF187 mRNA in HCCLM3, MHCC97-H, Huh7, PLC, SMMC-7721 and HepG2 cell lines; (C) Immunofluorescent staining for RNF187 in HCCLM3, Huh7, PLC and HepG2 cells. DAPI stain (blue) was used to identify nuclei (Scale bar: 200 μm); (D) RNF187 expression was down-regulated by pGPU6-GFP-vshRNA-RNF187s in HCCLM3, and up-regulated by pGPU6-RFP-cDNA-RNF187 in HepG2 cells, and #3 was validated for the most efficient interference of RNF187 by qRT-PCR and immunoblotting. (E) Cells with low RNF187 expression migrated slowly compared with those with high RNF187 expression at 48 h for wound channel closure. (F) The clone formation was elevated in cells with high level of RNF187. (G and H) Transwell assay showed that the numbers of invading cells in groups with low RNF187 expression were higher than those with high RNF187 expression, and the volume of the tumors derived from HCC isogenic cell lines was calculated in vivo for 6 weeks; Serial sections from mouse lung showed the metastasis ability of cancer cells expressing different RNF187 (Scale bar: 50 μm); and cell proliferation was positively associated with RNF187 expression in vitro. Magnification, ×200 (E and G) and ×100 (I).