Figure 2. Decrease TCTP level in normal state lung cells has little effect on reversion.

(A) Knockdown TCTP in A549 with siRNA (right), the clone number in soft agar. Only under the stressful condition (transfect with H1 parvovirus), TCTP knockdown can show a remarkable effect on A549. (B) Tumor development in nude mouse subcutaneously injected with A549 with different treatment. Tumor formation on a pair of mice with each genotype is shown on the left, and tumor volumes (mean ± SD) from each group of six mice during the period of 6 weeks after injection are plotted on the right. (C) the same pattern also can be observed in transwell assay. Data are shown as mean ± SD of three independent experiments. ^*P < 0.05, ^***P < 0.001 compared with control.