Table 1. Encapulation efficiency, mean particle diameter, and polydispersity index when one variable parameter was fixed.

Order of evaluation	Variable	Level	Encapulation efficiency (%)±S.D.	Mean particle diameter (nm) ±S.D.	Polydispersityindex±S.D.
1st	The proportion of GA to blank liposomes (w/w)	1:6	81.45±0.60	225.43±0.65	0.5033±0.0071
		1:8	83.24±0.92	209.13±2.44	0.4230±0.0030
		1:9	91.06±0.59	221.17±2.76	0.4223±0.0055
		1:10	78.64±1.07	227.47±2.33	0.4830±0.0020
2nd	The molar ratio of Gal to EPC	3 %	91.28±0.67	190.60±0.75	0.3850±0.0046
		5 %	85.52±0.62	187.17±0.85	0.3217±0.0068
		7 %	84.55±0.10	196.17±0.86	0.4023±0.0068
		9 %	80.92±0.57	199.73±0.38	0.4410±0.0030
3rd	The type of cryoprotectant	mannitol	78.32±0.48	221.50±0.98	0.5383±0.0067
		trehalose	77.88±0.31	218.27±0.97	0.4530±0.0030
		sucrose	91.28±0.03	232.70±0.66	0.4793±0.0025
		glucose	86.94±0.20	189.33±0.75	0.5080±0.0026
		glucose-mannitol	93.22±0.62	185.83±0.47	0.3457±0.0025
		glucose-trehalose	87.25±0.75	212.37±0.96	0.4150±0.0025
		sucrose-trehalose	87.71±0.73	212.20±0.80	0.4280±0.0044
		sucrose-glucose	84.55±0.64	234.63±0.61	0.5347±0.0020
		mannitol-trehalose	88.65±0.39	225.20±0.75	0.4660±0.0034
		mannitol-sucrose	91.47±0.57	193.43±0.87	0.3853±0.0025
4th	The proportion of cryoprotectant to EPC (w/w)	4:1	82.57±0.66	225.30±1.28	0.4677±0.0051
		6:1	84.79±0.63	210.00±0.78	0.3413±0.0045
		8:1	81.83±0.84	218.27±0.65	0.4617±0.0045
		10:1	81.48±0.75	211.07±0.78	0.3997±0.0035
		12:1	81.03±0.99	230.37±0.82	0.4593±0.0056
5th	The method of preparation	thin-film dispersion method	92.77±0.62	181.00±0.66	0.3740±0.0045
		ethanol injection method	81.71±0.95	188.50±0.75	0.4983±0.0040

Notes: Data shown represent the mean ± S.D.

Abbreviations: S.D., standard deviation.