Table 5. Intra-day and Inter-day accuracy and precision of the method for determination of GA in biological samples.
| Biological sample | QC (ng/mL) | Intra-day (n=6) | Inter-day (n=6) | ||||
|---|---|---|---|---|---|---|---|
| Mean concentration (ng/mL) ± S.D. | Accuracy (R.E.%) | Precision (R.S.D.%) | Mean concentration (ng/mL) ± S.D. | Accuracy (R.E.%) | Precision (R.S.D.%) | ||
| Plasma | 25.00 | 25.19 ± 1.02 | 0.75 | 4.06 | 26.28 ± 1.10 | 5.13 | 4.17 |
| 800.00 | 809.38 ± 24.21 | 1.17 | 2.99 | 822.24 ±16.57 | 2.78 | 2.02 | |
| 2000.00 | 1925.79 ± 139.23 | -3.71 | 7.23 | 2119.71 ± 124.08 | 5.99 | 5.85 | |
| Heart | 25.00 | 25.62 ± 1.01 | 2.48 | 3.96 | 26.68 ± 1.10 | 6.72 | 4.11 |
| 800.00 | 791.45 ± 39.25 | -1.07 | 4.96 | 838.11 ± 36.57 | 4.76 | 4.36 | |
| 2000.00 | 2051.49 ± 78.79 | 2.57 | 3.84 | 2190.51 ± 169.05 | 9.53 | 7.72 | |
| Liver | 25.00 | 25.41 ± 1.28 | 1.63 | 5.04 | 26.01 ± 1.86 | 4.02 | 7.14 |
| 800.00 | 825.17 ± 30.03 | 3.15 | 3.64 | 845.68 ± 31.87 | 5.71 | 3.77 | |
| 2000.00 | 1945.70 ± 70.76 | -2.71 | 3.64 | 2191.93 ± 112.10 | 9.60 | 5.11 | |
| Spleen | 25.00 | 25.47 ± 0.88 | 1.87 | 3.44 | 24.54 ± 1.56 | -1.83 | 6.37 |
| 800.00 | 779.13 ± 20.48 | -2.61 | 2.63 | 832.60 ± 23.45 | 4.08 | 2.82 | |
| 2000.00 | 2126.29 ± 209.11 | 6.31 | 9.83 | 2189.88 ± 157.19 | 9.49 | 7.18 | |
| Lung | 25.00 | 24.66 ± 1.04 | -1.37 | 4.21 | 25.63 ± 1.73 | 2.53 | 6.76 |
| 800.00 | 779.78 ± 26.37 | -2.53 | 3.38 | 787.26 ± 48.25 | -1.59 | 6.13 | |
| 2000.00 | 2049.59 ± 189.36 | 2.48 | 9.24 | 2142.81 ± 138.66 | 7.14 | 6.47 | |
| Kidney | 25.00 | 25.85 ± 1.28 | 3.40 | 4.95 | 26.28 ± 1.16 | 5.11 | 4.41 |
| 800.00 | 831.41 ± 28.78 | 3.93 | 3.46 | 853.46 ± 29.21 | 6.68 | 3.42 | |
| 2000.00 | 2070.00 ± 163.94 | 3.50 | 7.92 | 2178.93 ± 178.35 | 8.95 | 8.19 | |
Note: R.S.D. (%) = (S.D./mean concentration) *100 %; R.E. (%) = [(mean concentration/QC)-1] *100 %.
Abbreviations: GA, glycyrrhetinic acid; QC, quality control samples; R.E., relative error; R.S.D., relative standard deviation; S.D., standard deviation.