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. 2017 Oct 31;8(60):102119–102133. doi: 10.18632/oncotarget.22183

Figure 3. MiR-29b promoted EMT and regulated epigenetic changes in BC cells.

Figure 3

(a) Western blot analysis was performed to detect the expression of E-cadherin and Vimentin in MDA-MB-231 cells transfected with miR-29b inhibitor and MCF-7 cells transfected with miR-29b mimics. (b−c) An immunofluorescence assay was used to detect the expression level of E-cadherin and Vimentin in miR-29b inhibitor-transfected MDA-MB-231 cells or mimic-transfected MCF-7 cells. (d) The 5hmC level was detected in MDA-MB-231 cells transfected with miR-29b inhibitor and in MCF-7 cells transfected with miR-29b mimics. The immunofluorescence signal was quantified using densitometric scanning software, and the relative protein abundance was determined by normalization to the level of β-actin. All data are the means ± S.E.M; n = 3. *P < 0.05; **P < 0.01.