Skip to main content
. 2017 Nov 1;8(60):102176–102190. doi: 10.18632/oncotarget.22250

Figure 4. MIBE activates pluripotent transcription factors.

Figure 4

(A), Immunofluorescent staining shows increased c-Myc, Klf4, Oct4, and Sox2 in epithelial cells for colon biopsies from E. faecalis-colonized Il10-/- mice (lower panels, 20x) compared to sham-colonized mice (upper panels, 20x). Insets (100x) show differing patterns for these factors. (B), Western blots show increased c-Myc, Klf4, Oct4, and Sox2 expression in YAMC cells treated with 4-HNE for 1 hr (upper) after normalized to untreated control (lower). (C), Similarly, TNFα induces c-Myc, Klf4, Oct4, and Sox2 in these cells (upper). Lower panel, normalization of Western blots with β-actin and untreated control. All data represent mean ± SD for 3 independent experiments.