Figure 4.

BDNF signaling specifically induces the release of mRNAs from hnRNP K-containing complexes at synaptic sites. A, Effect of BDNF and PDGF on the interaction of GluA1, GluN1, and BDNF mRNAs with hnRNP K in synaptoneurosomes. The relative abundance of each mRNA coimmunoprecipitated with hnRNP K was evaluated by qPCR. Results are the mean ± SEM of four different experiments performed in independent preparations, and are expressed as fold change to control; *p < 0.05, **p < 0.01, as determined by one-way ANOVA followed by the Dunnett’s multiple comparison test. Activation of Akt and ERK1/2 by BDNF (B) and PDGF (C) in hippocampal synaptoneurosomes. Graphs represent the fold change in the levels of pAkt, pERK1, or pERK2 in hippocampal synaptoneurosomes stimulated with BDNF (50 ng/ml) or PDGF (20 ng/ml) for 10 min. Representative Western blotting images showing the results obtained for the phosphorylated proteins and tubulin under control conditions and following stimulation with BDNF or PDGF, as indicated. The results represent the quantification of six to nine experiments performed in independent preparations, and are expressed as fold change (mean ± SEM) to control; *p < 0.05, **p < 0.01, ***p < 0.001 as determined by the Student’s t test.