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. Author manuscript; available in PMC: 2019 Jan 1.

Published in final edited form as: Cell Signal. 2017 Nov 8;42:227–235. doi: 10.1016/j.cellsig.2017.11.002

Fig. 2 — Inosine analog NBMPR dose-dependently induces hA_2AR-mediated cAMP production in cellular and cell-free membrane assays.

CHO-hA_2AR cells were incubated with adenosine (control) or NBMPR with and without ADA (3 U/ml) and in the presence/absence of the hA_2AR-selective inverse agonist ZM 241385 for 10 min (A). Mean intracellular cAMP levels ± SEM of a representative experiment are shown (n=3; **, < 0.01 vs DMSO; ***, < 0.001 vs DMSO; θθθ, P < 0.001 vs without ADA; ###, p < 0.001 vs DMSO with ADA; τττ, < 0.001 vs without ADA and ZM 241385; ^^^, p < 0.001 vs with ADA but without ZM 241385). CHO-hA_2AR cell membranes were incubated with indicated concentrations of NBMPR and CGS 21680 (CGS) in the presence and in the absence of the A_2AR-selective inverse agonist ZM 241385 for 30 min (B). Mean cAMP production ± SEM of representative experiments are shown (n=6; ** and ***, p < 0.05 and p < 0.001 vs DMSO respectively; ^^ and ^^^, p < 0.05 and p < 0.001 vs without ZM 241385 respectively).

Fig. 2 — Inosine analog NBMPR dose-dependently induces hA_2AR-mediated cAMP production in cellular and cell-free membrane assays.

CHO-hA_2AR cells were incubated with adenosine (control) or NBMPR with and without ADA (3 U/ml) and in the presence/absence of the hA_2AR-selective inverse agonist ZM 241385 for 10 min (A). Mean intracellular cAMP levels ± SEM of a representative experiment are shown (n=3; **, < 0.01 vs DMSO; ***, < 0.001 vs DMSO; θθθ, P < 0.001 vs without ADA; ###, p < 0.001 vs DMSO with ADA; τττ, < 0.001 vs without ADA and ZM 241385; ^^^, p < 0.001 vs with ADA but without ZM 241385). CHO-hA_2AR cell membranes were incubated with indicated concentrations of NBMPR and CGS 21680 (CGS) in the presence and in the absence of the A_2AR-selective inverse agonist ZM 241385 for 30 min (B). Mean cAMP production ± SEM of representative experiments are shown (n=6; ** and ***, p < 0.05 and p < 0.001 vs DMSO respectively; ^^ and ^^^, p < 0.05 and p < 0.001 vs without ZM 241385 respectively).