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. Author manuscript; available in PMC: 2019 Jan 1.

Published in final edited form as: Cell Signal. 2017 Nov 8;42:227–235. doi: 10.1016/j.cellsig.2017.11.002

Fig. 3 — PAM of the A_2AR AEA061 enhances inosine-inducible, A_2AR-mediated cAMP production. CHO-hA_2aR cells were incubated with inosine (Ino; 1000 μM) and AEA061 (10 μM) in the presence and in the absence of ZM 241385 (100 nM) for 10 min (A). Mean cAMP levels ± SEM of a representative experiment is shown (n=3; ***, p < 0.001 vs DMSO; ###, < p < 0.001 vs inosine; &&&, p < 0.001 vs without ZM 241385). AEA061 increases E_max (B) and alters EC₅₀ (C) of inosine-mediated A_2AR activation. CHO-A_2AR cells were incubated with AEA061 and varying concentrations of inosine for 10 min. Mean E_max (B) and EC₅₀ (C) values of four experiments with SEM are shown (**, < 0.01 vs DMSO; # and ##, p < 0.05 and 0.01 vs without ADA pretreatment; ^^, p < 0.01 vs ADA pretreatment without subsequent ADA addition respectively).

Fig. 3 — PAM of the A_2AR AEA061 enhances inosine-inducible, A_2AR-mediated cAMP production. CHO-hA_2aR cells were incubated with inosine (Ino; 1000 μM) and AEA061 (10 μM) in the presence and in the absence of ZM 241385 (100 nM) for 10 min (A). Mean cAMP levels ± SEM of a representative experiment is shown (n=3; ***, p < 0.001 vs DMSO; ###, < p < 0.001 vs inosine; &&&, p < 0.001 vs without ZM 241385). AEA061 increases E_max (B) and alters EC₅₀ (C) of inosine-mediated A_2AR activation. CHO-A_2AR cells were incubated with AEA061 and varying concentrations of inosine for 10 min. Mean E_max (B) and EC₅₀ (C) values of four experiments with SEM are shown (**, < 0.01 vs DMSO; # and ##, p < 0.05 and 0.01 vs without ADA pretreatment; ^^, p < 0.01 vs ADA pretreatment without subsequent ADA addition respectively).