Table 5.
| a. Effects of 24 h, 48 h, 6 day exposures to TBT on IL-6 secretion from PBMCs. | ||||
|---|---|---|---|---|
| 24 h | Interleukin 6 secreted in pg/mL (mean±S.D.) | |||
|
| ||||
| [TBT] nM | F252 | F257 | F261 | F264 |
| 0 | 107735±4735 | 123182±4413 | 108921±458 | 46005±308 |
| 2.5 | 119503±1019+ | 157545±2460+ | 156436±1916+ | 109857±1151+ |
| 5 | 106508±3257 | 160273±656+ | 130376±688+ | 90252±1561+ |
| 10 | 89450±4474* | 158333±1001+ | 102073±6556 | 80770±2100+ |
| 25 | 1365±220* | 150697±105+ | 119042±2900+ | 86894±964+ |
| 50 | 100201±8444 | 172636±10351+ | 106618±315* | 83240±4790+ |
| 100 | 12540±1176* | 120030±1211 | 55527±793* | 90844±901+ |
| 200 | 1386±477* | ND* | ND* | ND* |
| 48 h | Interleukin 6 secreted in pg/mL (mean±S.D.) | |||
|---|---|---|---|---|
|
| ||||
| [TBT] nM | F252 | F257 | F261 | F264 |
| 0 | 86054±3721 | 159936±402 | 189796±3587 | 141±370 |
| 2.5 | 112033±8360+ | 177617±1486+ | 118288±5352* | 104303±210+ |
| 5 | 112825±3112+ | 199994±2634+ | 200393±321+ | 98768±3031+ |
| 10 | 114158±1589+ | 198545±1328+ | 192884±1895 | 94040±1152+ |
| 25 | 79846±6771 | 219299±7449+ | 211481±5805+ | 99091±1283+ |
| 50 | 10596±1016+ | 233965±1424+ | 205656±8477 | 159333±1266+ |
| 100 | ND* | 155183±1086+ | 98358±3466* | 147333±1748+ |
| 200 | ND* | 2371±822* | ND* | 52990±573+ |
| 6 day | Interleukin 6 secreted in pg/mL (mean±S.D.) | |||
|---|---|---|---|---|
|
| ||||
| [TBT] nM | F252 | F257 | F261 | F264 |
| 0 | 143040±5912 | 164368±443 | 170226±2050 | 66006±1860 |
| 2.5 | 124754±5201* | 182069±2296+ | 165820±2790 | 77252±1936+ |
| 5 | 131230±4148* | 149609±939* | 199386±1616+ | 81020±2811+ |
| 10 | 150126±4018 | 197011±2704+ | 170226±11833 | 107049±1158+ |
| 25 | 156564±12441 | 212598±522+ | 172835±9901 | 97831±611+ |
| 50 | 150583±1126 | 217287±1795+ | 173241±8388 | 131513±1254+ |
| 100 | 94735±5760* | 154345±1202* | 38864±2611* | 228035±1313+ |
| 200 | 2011±695* | 7816±80* | ND* | 11223±100* |
| b. Effects of 24 h, 48 h, 6 day exposures to TBT on IL-6 secretion from PBMCs plus Granulocytes. | ||||
|---|---|---|---|---|
|
| ||||
| 24 h | Interleukin 6 secreted in pg/mL (mean±S.D.) | |||
| [TBT] nM | F252 | F257 | F261 | F264 |
| 0 | 24852±1323 | 37539±1932 | 26205±352 | 3124±3 |
| 2.5 | 30787±1395+ | 44261±1248+ | 18660±906* | 3107±16 |
| 5 | 38369±748+ | 46178±1355+ | 24221±203* | 3317±22+ |
| 10 | 37506±157+ | 55233±1673+ | 26693±593 | 3155±17 |
| 25 | 47101±460+ | 59678±966+ | 24774±982 | 3191±36 |
| 50 | 48068±1024+ | 66622±3007+ | 26985±1150 | 3514±69+ |
| 100 | 68199±958+ | 77344±2115+ | 40481±2245+ | 4951±47+ |
| 200 | 20591±432* | 22733±300* | 29652±1043+ | 4079±18+ |
| 48 h | Interleukin 6 secreted in pg/mL (mean±S.D.) | |||
|---|---|---|---|---|
|
| ||||
| [TBT] nM | F252 | F257 | F261 | F264 |
| 0 | 8475±122 | 17469±302 | 3058±96 | 1085±18 |
| 2.5 | 9798±245+ | 23335±325+ | 3991±109+ | 1112±28 |
| 5 | 13232±1047+ | 24021±1180+ | 3800±15+ | 1183±30+ |
| 10 | 10586±367+ | 35240±198+ | 3823±81+ | 1216±7+ |
| 25 | 17818±316+ | 23488±540+ | 3980±122+ | 1385±45+ |
| 50 | 17101±364+ | 42078±994+ | 4429±48+ | 1642±40+ |
| 100 | 28636±786+ | 52935±293+ | 4577±166+ | 3264±32+ |
| 200 | 8182±121* | 15926±198* | 2188±62* | 2387±5+ |
| 6 day | Interleukin 6 secreted in pg/mL (mean±S.D.) | |||
|---|---|---|---|---|
|
| ||||
| [TBT] nM | F252 | F257 | F261 | F264 |
| 0 | 280±34 | 1063±21 | 13±0 | 86±3 |
| 2.5 | 219±4 | 1080±187 | 16±1+ | 345±23+ |
| 5 | 375±14+ | 1159±7+ | 16±2 | 94±12 |
| 10 | 278±51 | 1782±18+ | 26±0+ | 64±5* |
| 25 | 531±4+ | 2380±7+ | 30±0+ | 117±12+ |
| 50 | 924±27+ | 3718±15+ | 35±2+ | 650±3+ |
| 100 | 3318±51+ | 7225±28+ | 97±2+ | 1581±31+ |
| 200 | 355±37 | 429±37* | 65±2+ | 347±16+ |
| c. Effects of 24 h, 48 h, 6 day exposures to TBT on IL-6 secretion from Granulocytes. | ||||
|---|---|---|---|---|
| 24 h | Interleukin 6 secreted in pg/mL (mean±S.D.) | |||
|
| ||||
| [TBT] nM | F252 | F257 | F261 | F264 |
| 0 | 122±2 | 137±11 | 785±53 | 137±13 |
| 2.5 | 882± 41+ | 507±3+ | 1214±11+ | 460±9+ |
| 5 | 1044±13+ | 300±5+ | 1238±42+ | 326±25+ |
| 10 | 963±30+ | 313±20+ | 1515±25+ | 309±23+ |
| 25 | 315±20+ | 219±34+ | 948±10+ | 209±2+ |
| 50 | 299±35+ | 161±1 | 1304±21+ | 516±19+ |
| 100 | 229±23+ | 162±6+ | 1306±17+ | 339±16+ |
| 200 | ND* | ND* | 206±22* | ND* |
| 48 h | Interleukin 6 secreted in pg/mL (mean±S.D.) | |||
|---|---|---|---|---|
|
| ||||
| [TBT] nM | F252 | F257 | F261 | F264 |
| 0 | 6±6 | 110±15 | 87±14 | 1292±25 |
| 2.5 | 733±20+ | 949±12+ | 382±0+ | 1329±38 |
| 5 | 1635±15+ | 885±35+ | 305±27+ | 1428±42+ |
| 10 | 1380±15+ | 1260±12+ | 297±13+ | 1474±9+ |
| 25 | 365±6+ | 1110±51+ | 201±8+ | 1710±62+ |
| 50 | 394±19+ | 635±25+ | 203±2+ | 2068±55+ |
| 100 | 274±5+ | 163±13+ | 80±8* | 4328±45+ |
| 200 | ND* | ND* | ND* | 3106±6+ |
| 6 day | Interleukin 6 secreted in pg/mL (mean±S.D.) | |||
|---|---|---|---|---|
|
| ||||
| [TBT] nM | F252 | F257 | F261 | F264 |
| 0 | 9±0 | 5±1 | 6±1 | 4±1 |
| 2.5 | 25±1+ | 48±1+ | 29±1+ | 14±+ |
| 5 | 22±1+ | 53±1+ | 9±2 | 7±1+ |
| 10 | 12±1+ | 18±1+ | 9±1+ | 9±2+ |
| 25 | 10±0+ | 8±0+ | 8±1 | 8±1+ |
| 50 | 10±1 | 9±1+ | 7±2 | 8±1+ |
| 100 | 9±1 | 8±1+ | 10±1+ | 6±1 |
| 200 | 8±0* | 4±0 | 8±1+ | 4±1 |
Values are mean±S.D. of triplicate determinations.
*
Indicates a significant decrease in secretion and
+
indicates a significant increase in secretion compared to control cells (cells treated with vehicle alone), p<0.05 ND = not detectable.