. Author manuscript; available in PMC: 2019 Feb 1.

Published in final edited form as: J Appl Toxicol. 2017 Aug 24;38(2):201–218. doi: 10.1002/jat.3514

Table 8.

a. Effects of 24 h, 48 h, 6 day exposures to DBT on IL-6 secretion from PBMCs.
24 h	Interleukin 6 secreted in pg/mL (mean±S.D.)

[DBT] µM	F285	F290	F294	F299
0	34255±142	14998±986	32382±357	95826±2410
0.05	63720±1078⁺	61442±2081⁺	92678±862⁺	112870±348⁺
0.1	60002±523⁺	65886±1367⁺	53085±3654⁺	118145±724⁺
0.25	76453±765⁺	69788±4877⁺	63289±641⁺	119362±362⁺
0.5	68152±745⁺	53195±3027⁺	61937±861⁺	61681±3093^*
1	14593±438^*	16405±1398	13622±735^*	17275±703^*
2.5	ND^*	ND^*	ND^*	ND^*
5	ND^*	ND^*	ND^*	ND^*

48 h	Interleukin 6 secreted in ng/mL (mean±S.D.)

[DBT] µM	F285	F290	F294	F299
0	42735±177	53716±1836	30850±33	97222±962
0.05	61846±122⁺	76212±1266⁺	45764±344⁺	148278±631⁺
0.1	70898±912⁺	87289±884⁺	43992±772⁺	157389±1549⁺
0.25	88325±798⁺	108349±711⁺	63154±343⁺	124000±2333⁺
0.5	91577±266⁺	91289±1299⁺	52811±348⁺	83444±1678^*
1	15717±1110^*	49716±872^*	27078±788^*	23944±1798^*
2.5	ND^*	ND^*	50±216^*	ND^*
5	ND^*	ND^*	ND^*	ND^*

6 day	Interleukin 6 secreted in ng/mL (mean±S.D.)

[DBT] µM	F285	F290	F294	F299
0	3922±9	11020±543	35576±77	115832±599
0.05	14426±4⁺	18824±471⁺	50986±239⁺	152672±2344⁺
0.1	19597±8⁺	24039±245⁺	58209±77⁺	168474±3388⁺
0.25	41418±6⁺	37294±1387⁺	46682±674⁺	139042±905⁺
0.5	32052±1⁺	42980±1682⁺	30716±1216^*	89264±1864^*
1	ND^*	15294±204⁺	7323±155^*	21314±226^*
2.5	ND^*	ND^*	116±51^*	ND^*
5	ND^*	ND^*	ND^*	ND^*

b. Effects of 24 h, 48 h, 6 day exposures to DBT on IL-6 secretion from PBMCs plus Granulocytes.
24 h	Interleukin 6 secreted in pg/mL (mean±S.D.)

[DBT] µM	F285	F290	F294	F299
0	8587±89	8593±60	12411±381	15883±675
0.05	8138±302^*	10178±136⁺	15173±84⁺	18437±737⁺
0.1	12487±328⁺	9162±324	14586±300⁺	18727±658⁺
0.25	16162±363⁺	11058±195⁺	14788±307⁺	20621±407⁺
0.5	17618±430⁺	10477±130⁺	15558±28⁺	21298±388⁺
1	9364±102⁺	7475±34^*	7244±146^*	17080±404
2.5	265±25^*	520±17^*	0±18^*	2804±60^*
5	ND^*	ND^*	ND^*	267±92^*

48 h	Interleukin 6 secreted in pg/mL (mean±S.D.)

[DBT] µM	F285	F290	F294	F299
0	5797±38	6346±13	11944±111	11795±238
0.05	6270±58⁺	9284±64⁺	15322±317⁺	13838±531⁺
0.1	6792±88⁺	7465±315⁺	14925±466⁺	15217±419⁺
0.25	9783±17⁺	11645±78⁺	17219±17⁺	19809±226⁺
0.5	10257±307⁺	11280±107⁺	15552±110⁺	20011±851⁺
1	7253±34⁺	9277±30⁺	8777±93^*	16104±2294
2.5	394±11^*	95±16^*	ND^*	1150±264^*
5	215±45^*	ND^*	ND^*	ND^*

6 day	Interleukin 6 secreted in pg/mL (mean±S.D.)

[DBT] µM	F285	F290	F294	F299
0	21±10	30±10	1843±18	49±4
0.05	161±4⁺	498±12⁺	1845±49	348±244
0.1	159±13⁺	531±9⁺	1991±36	656±166⁺
0.25	359±15⁺	260±6⁺	2699±16⁺	456±37⁺
0.5	550±15⁺	316±18⁺	2638±22⁺	321±26⁺
1	99±10⁺	108±5⁺	1020±35^*	107±8⁺
2.5	ND^*	ND^*	ND^*	37±5^*
5	ND^*	ND^*	ND^*	26±54

c. Effects of 24 h, 48 h, 6 day exposures to DBT on IL-6 secretion from Granulocytes.
24 h	Interleukin 6 secreted in pg/mL (mean±S.D.)

[DBT] µM	F285	F290	F294	F299
0	54±2	46±2	16±1	148±0
0.05	89±4⁺	71±10⁺	16±4	197±8⁺
0.1	72±3⁺	65±8⁺	10±1^*	254±9⁺
0.25	92±2⁺	93±25	17±3	22±1^*
0.5	79±1⁺	65±2⁺	1±2^*	252±3⁺
1	19±1^*	15±1^*	0±1^*	162±4⁺
2.5	ND^*	ND^*	ND^*	17±0^*
5	ND^*	ND^*	ND^*	8±0^*

48 h	Interleukin 6 secreted in pg/mL (mean±S.D.)

[DBT] µM	F285	F290	F294	F299
0	35±10	58±2	36±1	149±4
0.05	34±3	57±9	34±0^*	143±1
0.1	33±5	59±4	31±2^*	136±2^*
0.25	44±3⁺	78±6⁺	45±3⁺	221±8⁺
0.5	32±1^*	70±7	30±3^*	231±3⁺
1	5±2^*	20±0^*	6±1^*	131±9^*
2.5	ND^*	ND^*	ND^*	ND^*
5	ND^*	ND^*	ND^*	ND^*

6 day	Interleukin 6 secreted in pg/mL (mean±S.D.)

[DBT] µM	F285	F290	F294	F299
0	ND	6±0	ND	4±0
0.05	ND	22±1⁺	ND	9±1⁺
0.1	ND	23±4⁺	ND	5±1
0.25	ND	18±4⁺	ND	6±1
0.5	ND	10±3	ND	4±0
1	14±2⁺	7±3	ND	7±1
2.5	ND	4±2	ND	7±1⁺
5	ND	ND^*	ND	6±1⁺

Values are mean±S.D. of triplicate determinations.

Indicates a significant decrease in secretion and

⁺

indicates a significant increase in secretion compared to control cells (cells treated with vehicle alone), p<0.05 ND = not detectable.