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. 2017 Dec 11;7:501. doi: 10.3389/fcimb.2017.00501

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Copyright © 2017 Kintner, Moore, Whittimore, Butler and Hall.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Impact of Wnt inhibition on chlamydial infection in IK/SHT-290 co-culture. Replicate IK/SHT-290 co-cultures were exposed to DMSO or the Wnt inhibitor, IWP2, prior to and following C. trachomatis infection. At 44 hpi, the infected IK monolayers and SHT-290 monolayers were collected and processed for (A) percent infectivity analysis, (B) EB titer assay, (C) inclusion size measurement, (D) and Western blot analysis of β-catenin accumulation. The data shown represent three independent experiments with 3 biological replicates per experiment. Comparison of means was performed using a 2-sample T-test for independent samples. P ≤ 0.05 were considered statistically significant. ^*Indicates a significant difference (p ≤ 0.05) from the DMSO control.