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. 2017 Dec 11;5:77. doi: 10.3389/fbioe.2017.00077

Figure 3.

Figure 3

Immunocytochemical staining of skeletal stem cells (SSCs) seeded on ethylene oxide sterilization (EOS) titanium templates and tissue culture plastic (TCP) at day 14. Confocal microscopy images of the 90-µm pore surface of the titanium template using (A) reflectance mode (scale bar represents 100 µm), (B) normal mode, showing HCS CellMask™ Deep Red stained SSCs (magenta) seeded on the 90-µm pore surface of the titanium template (scale bar represents 100 µm). (C) Cytoskeletal filament orientation and cell distribution as demonstrated by F-actin (TRITC—red), α-tubulin (AlexaFluor-488—green), and nuclei (DAPI—blue) stains, scale bar represents 200 µm. (D) F-actin, α-tubulin, and nuclei appearance of SSCs directly in contact with the titanium surface, and SSCs growing within the 90-µm pores (white dotted circles). Scale bar represents 100 µm. (E) Virtual cross-sectional view of (D). The solid blue axis delineates the template surface. Scale bar represents 50 µm. (F,G) Cytoskeletal filament orientation of SSCs cultured on TCP in basal media and osteogenic media, respectively. Scale bar represents 200 µm.