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. 2017 Dec 11;5:77. doi: 10.3389/fbioe.2017.00077

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Copyright © 2017 Tang, Yang, Ou and Oreffo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative expressions of Runx2, Col1a1, alkaline phosphatase (ALP), osteopontin (OPN), osteocalcin (OCN), and β-actin at days 7, 14, and 21. Skeletal stem cells (SSCs) on TCP were cultured in basal (in blue) and osteogenic (in red) media. SSCs on ethylene oxide sterilization (EOS) templates were cultured in basal media (in gray). Relative expression of each gene marker at all time-points was normalized against the relative expression of the same marker from basal day 7 samples (*p < 0.05, ^ǂp < 0.01, ^§p < 0.001).