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. 2017 Dec 15;7:17617. doi: 10.1038/s41598-017-18061-y

Figure 5.

Figure 5

HSF1 inhibition reduces FN1 expression. HEK293FT cells transfected with the pGL4-pFN1 promoter reporter plasmid and transfection efficiency control pEGFP were treated with or without the HSF1 inhibitor KRIBB11 in the (A) absence or (B) presence of geldanamycin (GA) for 24 hours. Luminescence values are relative to GFP fluorescence and data shown are representative of 3 biological replicates per treatment with 3 technical replicates per transfection. Statistical analysis was conducted by comparing the treated cells to the DMSO control using one way ANOVA with Bonferroni post-test (**p < 0.001). (C) Hs578T cells were treated for 24 hours with GA (100 nM), Rapamycin (50 nM), both or the DMSO vehicle control. Total RNA was extracted and qPCR was conducted using PPIA as a reference gene. Data shown are average mRNA abundance (ΔCt) relative to the DMSO vehicle control from 3 biological experiments conducted in technical triplicates. Statistical analysis was conducted using one way ANOVA with Bonferroni post-test (***p < 0.001).