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. 2017 Dec 15;7:17617. doi: 10.1038/s41598-017-18061-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Geldanamycin induced up-regulation of FN1 is dependent on heat shock elements in the promoter. (A) The cloned FN1 promoter sequence annotated with putative HSEs and key transcription motifs. The CCAAT box (−334 bp) and TATA box (−27 bp) are identified relative to the TSS ( + 1 bp) and start codon ( + 200 bp)⁷⁷. (B) Two truncations of the FN1 promoter were made by PCR amplification and validated by Sanger sequencing. (C) HEK293FT cells were transfected with pGL4-pFN1, pGL4-pFN1₋₈₁₀, pGL4-pFN1₋₃₈₀ or empty pGL4.17 and transfection efficiency control pCAG-HRP-TM and treated with 100 nM GA or DMSO. Relative luciferase values are normalised to the DMSO treated pGL4-pFN1 and are representative of 6 independent transfections per treatment with 3 technical replicates per transfection. Statistical analysis was conducted in comparison to the wild type untreated pGL4-pFN1 treatment using one way ANOVA with Bonferroni post-test (***p < 0.001).