Figure 1.

DHC-induced hypothermia significantly reduces cortical injury after one month post-stroke. (A) Schemes of experimental procedure. Ischemia was induced for 120 minutes followed by 90 minutes of reperfusion with heat support. Either DHC or vehicle was infused by an osmotic pump for 8 hours starting at 90-minute after reperfusion. Behavioral tests were performed on post-stroke day 3, 7, 14, 21 and 28. On post-stroke day 30, mice were sacrificed and brain samples were collected for further histology analysis (Nissl staining and immunostaining) in all groups. (B) Representative images of brain section at +1 mm, 0 mm, −1 mm and −2 mm from bregma stained by Nissl stain. Full section high resolution images were generated by stitching together a total of 400 confocal images (20× magnification). Scale bar = 1 mm. (C) Summary of cortical infarct area. Cortical infarct% = [(contralateral cerebral cortex area − ipsilateral cerebral cortex area + infarcted area)/contralateral cerebral cortex area] × 100. (t test, *p < 0.05, n = 10 in Stroke/vehicle and 8 in Stroke/DHC, data are expressed as mean ± SEM).