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. 2017 Dec 15;7:17624. doi: 10.1038/s41598-017-17912-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Release of biocytin from the Biocage after 24 hour in vivo implantation. (a–c) Biocages were filled with biocytin in 0.35% agarose and capped. Biocytin labeling is visible in both the Biocage and areas immediately surrounding the Biocage (a, green puncta). Cells are only observed surrounding the enclosed Biocage (b, counterstain with DAPI for nuclei). (c) Is a higher magnification of the area denoted by the yellow box in (a and b) showing the biocytin released from the Biocage. (d–f) Control Biocage only filled with 0.35% agarose and capped. No puncta is observed within or outside of the Biocage (d). Cells are also not observed within the Biocage (e). (f) is a higher magnification of the area denoted in (d and e). Scale bar = 100 µm.