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. 2017 Dec 15;7:17624. doi: 10.1038/s41598-017-17912-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vivo implantation of the Biocage. (a and b) Uncapped and unfilled Biocages implanted into the cortex after 24 hour implantation in vivo. Biocages that are implanted more cortically are directly visible under light (a) and fluorescent (b) dissecting microscopy. (c–h) Coronal brain section showing implanted Biocage within the mouse hippocampus. (f–g) are high magnification images of (c–e), respectively. Implanted Biocages were cryosectioned directly and is visible under light (c,f) and fluorescence (d and e, g and h) microscopy. Fluorescence imaging of DAPI staining (e,h) shows tissue entering through the uncapped top; however, no cells were observed through the pores along the side wall (e,h). Scale bars = 300 µm.