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. 2017 Dec 15;7:17652. doi: 10.1038/s41598-017-17389-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Multivariate analysis (OPLS-DA) of CSF lipids from patients with ALS (n = 40) revealed 16 lipids involved in survival. (A) Score scatter plot. Blue dots: ALS patients with survival > median; green dots: ALS patients with survival < median. X-axis and Y-axis represent score vectors summarizing all the variables entering the analysis: t1 and to1 (B) Loading scatter plot. Variables near each other are positively correlated; variables opposite to each other are negatively correlated. Variables closer to dots corresponding to “higher survival” or “lower survival” (i.e. with the largest absolute loading values) are higher in the corresponding populations. Lipids from the same family are represented with the same color.