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. 2017 Dec 11;7:503. doi: 10.3389/fcimb.2017.00503

Figure 2.

Figure 2

In vitro invasion and proliferation of gapA, gapA-complemented, and wt FSC200 in bone marrow macrophages (BMMs) (A) and A549 cells (B). The cells were infected at MOI of 50:1 (BMM) or 200:1 (A549) with the indicated strains. BMMs were harvested at 1, 6, 12, 24, and 48 h and A549 at 4, 24, and 48 h post-infection. The numbers of bacteria recovered from the cells were counted as cfu. The data represent means ± SD of three independent experiments performed in triplicate. (C) Viability of cells and induction of cytotoxicity determined in BMMs infected with gapA mutant or wild-type FSC 200 strains. At 2, 24, and 48 h the cells were assayed using RealTime-Glo™ MT Cell Viability Assay kit and CellTox™ Green Cytotoxicity Assay kit (Promega). Data are means ± SD of triplicate samples and the results shown are representatives of three independent experiments. Asterisks indicate statistically significant differences; **P < 0.01; ***P < 0.001 (comparing gapA with the wild-type FSC200 strain).