Table 1.
Bacterial strains and plasmids used in this study.
| Strain/Plasmid | Genotype/Phenotype | Source/References |
|---|---|---|
| F. tularensis | ||
| FSC200 | F. tularensis subsp. holarctica; clinical isolate | Francisella strain collection Johansson et al., 2000 |
| dsbA | ΔFTS_1067/FSC200 | Straskova et al., 2009 |
| gapA | ΔFTS_1117/FSC200 | This study |
| gapA-complemented | ΔFTS_1117/FSC200::FTS_1117 | This study |
| E. coli | ||
| Top10 | F−mcrA Δ(mrr-hsdRMS-mcrBC), ϕ80lacZΔM15 ΔlacX74 recA1 deoR araD139 (Δara-leu)7697 galU galK rpsL (Smr) endA1 nupG | Invitrogen |
| S17-1λpir | recA, thi, pro, hsdR−M+, <RP4:2-Tc:Mu:Km:Tn7>TpR, SmR | Simon et al., 1983 |
| Plasmids | ||
| pCR®4.0-TOPO | TOPO-cloning vector. AmpR, KmR | Invitrogen |
| pDM4 | Suicide plasmid. sacB; mobRP4; oriR6K; CmR | Milton et al., 1996 |