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Sequences of primers used for creation of F. tularensis FSC200 gapA deletion mutant.
| Gene | Primer designation | 5′–3′ Sequence |
|---|---|---|
| gapA (FTS_1117) | A | CTCGAGTATATAGCTTCGCAATTGAGTAA |
| B | GCTCCGAAGTAACCATTAATTGCAACTCTCATTTT | |
| C | GCAATTAATGGTTACTTCGGAGCTCTATAAACA | |
| D | ACTAGTATCTCATCCGCAACAACATAG |
Restriction sites for selected endonucleases on primers A and D are in italic; complementary parts of primer B and C are underlined.
