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. 2017 Dec 11;8:2101. doi: 10.3389/fpls.2017.02101

FIGURE 2.

FIGURE 2

Sepal Yellowing of ag-1 and Ler-0 flowers. (A) Ler-0 inflorescence. (B) ag-1 inflorescence. (C) Chlorophyll concentration of Ler-0 and ag-1 sepals obtained from outermost buds. (D) Percentage chlorophyll of Ler-0 and ag-1 sepals at flower position 2 (indicated by white arrows in Figures 1C,D) compared with that in sepals from unopened flowers of the outer whorl shown here in (A,B), indicated by white arrows. Sepal chlorophyll percentage was calculated by comparing the chlorophyll concentration of sepals from flowers at position 2 to that in sepals from unopened flowers. Plants were grown under long-day conditions for 8 weeks as described in Section Materials and methods. (E) 8-week-old ag-1 plants with un-opened flowers. (F) Position of flowers showing yellowing and abscission in 8 week-old-ag-1 plants with opened and un-opened flowers. 8-week-old ag-1 plants with un-opened flowers were grown in a growth chamber with16-h-light/8-h-dark cycle, 100 μmol m-2 s-1 white light, and 60% relative humidity. The flowers are numbered according to the numbering system of Bleecker and Patterson (1997). The error bars indicate standard error of the means of six biological replicates. Indicates statistically significant (p < 0.05) between the wild type (Ler-0) and ag-1 using Student’s t-test.