FIGURE 4.

Exogenous application of MeJA to ag-1 flowers. (A) Mock- and 100 μM MeJA-treated ag-1 plants. Plants were grown under long-day conditions for 6 weeks and sprayed once a day with mock or MeJA solutions for 3 days. Flower buds with visible white petals were tagged with a cotton thread at day 0 of the MeJA or mock treatments and inflorescences were photographed after 3 days of treatment. (B) Flowers of mock- and 100 μM MeJA-treated ag-1 inflorescences were detached and photographed. (C) Percentage sepal yellowing of Mock- and MeJA-treated ag-1 and Ler-0 flowers. (D) Percentage sepal abscission of Mock- and MeJA-treated ag-1 and Ler-0 flowers. White arrow indicates floral bud at stage 13 (Alvarez-Buylla et al., 2010), yellow arrows indicate flowers with senesced sepals, and red arrow indicates a flower with abscised sepals. T represents the tagged flower, T^-1 and T^-2 represent flowers younger than T, T⁺¹ and T⁺² represent flowers older than T. 0% datapoints are placed slightly above the X-axis. Statistical differences between mock treated wild type and mutants (indicated by ^∗) and mock- and MeJA-treated mutant plants (indicated by ^#) were calculated according to the Wilson score confidence limits for a percentage as calculated in Agresti and Coull, 1998 (Supplementary Table S2). The photographs shown are representatives of three (C,D; MeJA treated T⁺¹ and T⁺²) or four to six biological replicates. Sen, Senescence; Abs, Abscission, n/a, not applicable.