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. 2017 Dec 11;8:2101. doi: 10.3389/fpls.2017.02101

FIGURE 5.

FIGURE 5

Exogenous application of MeJA to Col-0, dad1 and dde2 flowers. (A) Col-0, dad1 and dde2 plants were grown for 8 weeks and photographed. Mock and MeJA treated dad1 plants were grown for 6 weeks and flower buds with visible white petals were tagged with a cotton thread at day 0 of MeJA or mock treatment. Plants were sprayed once a day with a mock solution or MeJA for 3 days. Inflorescences were photographed after 3 days of treatment. (B) Detached flowers of Mock- and MeJA-treated dad1 inflorescences. (C) Percentage sepal yellowing of Mock- and MeJA-treated Col-0, dad1 and dde2 flowers. (D) Percentage sepal abscission of Mock- and MeJA-treated Col-0, dad1 and dde2 flowers. (E) dad1 inflorescences showing hand-pollinated flowers developing into siliques (white and black arrows). The white arrow in (A) indicate a floral bud at stage 13 according to Alvarez-Buylla et al. (2010), yellow arrows indicate flowers with senesced sepals, red arrows indicate flowers with abscised sepals. T represents the tagged flower, T-1 and T-2 represent flowers younger than T, T+1 and T+2 represent flowers older than T. The numbers in (A) represent the flowering position according to the numbering system of Bleecker and Patterson, 1997. The photographs shown are representatives of four to six biological replicates. Sen, Senescence; Abs, Abscission, 0% datapoints are placed slightly above the X-axis. Statistical differences between mock treated wild type and mutants (indicated by ∗) and mock- and MeJA-treated mutant plants (indicated by #) were calculated according to the Wilson score confidence limits for a percentage as calculated in Agresti and Coull (1998) (Supplementary Table S2).