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. 2017 Dec 15;7:17626. doi: 10.1038/s41598-017-17977-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescence microscopy of polyubiquitinated proteins in MPM cell lines. Cells were stained with FK2 antibody, which specifically recognizes poly-ubiquitin chains, and analyzed by confocal microscopy in three independent experiments. Nuclei labelled red by propidium iodide (PI); size bar: 50 μm lower magnification (left) and 10 μm higher magnification (right). Representative images. Cell area (µ²), mean ± St. dev.: MM98 108 ± 11, REN 117 ± 9, MMB 93 ± 12, MSTO-211H 110 ± 8.