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. 2017 Dec 15;7:17654. doi: 10.1038/s41598-017-17709-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RV’s cellular receptor JAM-A is present on the BV envelope. (a) Western blot analysis of Sf9 cells infected with BV^JAM. The blot was reacted with mAb α-HA followed by antibody-alkaline phosphatase, to detect the presence of JAM-A in infected cells. (b–d) Electron microscopic images of BV^JAM, negatively stained using uranyl acetate and incubated with mouse mAb α-JAM-A (i = sc-53623, ii = ab17261) followed by α-mouse antibody tagged with 10-nm colloidal gold. (b) Example of a BV^JAM field on grid showing low background labelling. (c,d) Enlargements of BV^JAM virions bearing respectively a single (c) or multiple (d) gold grains.