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. 2017 Dec 11;8:1776. doi: 10.3389/fimmu.2017.01776

Figure 2.

Figure 2

CD8+ T-cell responses against cytomegalovirus (CMV) peptides restricted by HLA-Cw*0702 are not cross-reactive, and their aggregate response represents a large percentage of the total CD8+ T-cell repertoire within older donors. (A) CMV-specific T-cell responses to different peptides do not show evidence of cross reactivity. PBMC from a CMV-seropositive donor aged 81 years was stimulated with either FRC (left), CRV (middle), LSE peptide (right), or all three peptides (far right) before intracellular cytokine staining for IFN-γ (top row) and TNF-α production (bottom row). (B) Distribution of individual peptide-specific CD8+ T-cell responses producing TNF-α within 16 donors. The TNF-α response to each peptide stimulation is shown as a percentage of the total CD8+ T-cell repertoire within individual donors (y-axis). The age of the donor in years is shown on the x-axis. One donor is included at subsequent time points (88 and 91 years) indicated by *. (C) Total magnitude of CMV-specific CD8+ T-cell response restricted by HLA-Cw*0702 within individual donors. Each circle represents the aggregate of the FRC, CRV, and LSE-specific CD8+ T-cell responses within individual donors as identified in panel (A). White circles represent donors <70 years of age, and black circles represent donors >70 years of age. Lines represent mean, and significant difference was determined using Mann–Whitney tests in GraphPad Prism 6 (***p < 0.001).