Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Dec 15;7:17656. doi: 10.1038/s41598-017-18024-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Chronic intracerebral administration of extracellular cGMP increases membrane expression of the GluA1 (A) and decreases GluA2 (B) subunits of AMPA receptors in cerebellum in vivo. Membrane expression of NR2A in the same samples is not affected by extracellular cGMP (D). For one group of rats the osmotic pumps were filled with 240 µM cGMP in sterile saline (cGMP) and for the other group with the vehicle solution, sterile saline (veh). These pumps released 0.25 μL per hour during 28 days. Cerebellum of rats was dissected and membrane expression of each subunit was analyzed using the BS3 crosslinker procedure as described in Methods. (C) Monomeric and cross-linked bands of GluA2 and actin from western blot are shown. Values are expressed as percentage of control rats (vehicle) and are the mean ± SEM of 16–19 rats per group. The unpaired Student’s t-test was performed. Values significantly different are indicated by asterisks *p < 0.05, **p < 0.01.