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. 2017 Dec 15;7:17656. doi: 10.1038/s41598-017-18024-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Addition of extracellular cGMP to cerebellar slices modulates membrane expression of AMPA receptor subunits and phosphorylation of Ser831 of GluA1 and Ser880 of GluA2. Exogenous cGMP (40 nM) was added to freshly isolated cerebellar slices. Membrane expression of GluA1 (A and B) and GluA2 (C and D) subunits were analyzed by BS3 crosslinking method (A and C) and by biotinylation assay (B and D) and their phosphorylation at Ser831 (E), Ser845 (F) and Ser880 (G), respectively, were analyzed as described in Methods. Values are expressed as percentage of basal and are the mean ± SEM of 20–30 rats. The unpaired Student’s t-test was performed. Values significantly different are indicated by asterisks *p < 0.05, **p < 0.01, ***p < 0.001.