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. 2017 Dec 15;7:17656. doi: 10.1038/s41598-017-18024-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Blocking glycine receptors with strychnine induces the same the effects as extracellular cGMP on phosphorylation at Ser831 of GluA1 and at Ser880 of GluA2 subunits but not at Ser845 of GluA1. Strychnine (75 µM), an antagonist of glycine receptor, was added to cerebellar slices and phosphorylation at Ser831 (A), Ser880 (B) and Ser845 (C) were analyzed as described in Methods. Values are expressed as percentage of basal and are the mean ± SEM of 8–16 rats. The unpaired Student’s t-test was performed, Values significantly different from basal are indicated by asterisks *p < 0.05, ***p < 0.001.