Skip to main content
. 2017 Dec 11;8:269. doi: 10.3389/fendo.2017.00269

Figure 5.

Figure 5

Both GnRH2 and GnRHR2 are abundantly produced within the porcine testis. (A) GnRH2 levels were determined via ELISA of homogenates from the hypothalamus, anterior pituitary gland, and testis of males (n = 6). a,bBars with alternate letters differ (P < 0.0001). (B) Representative western blot of anterior pituitary gland and testicular tissue from boars (n = 5) using an antibody directed against GnRHR2 (upper panel). Immunoblot quantification revealed a difference in GnRHR2 protein levels between tissue types (*P < 0.0001; lower panel). (C) GnRH2 is primarily localized to the tubular compartment of the porcine testis. Representative immunofluorescence image (400×; merge of fluorescence and transmitted light) of porcine testes (n = 5) using an antibody directed against GnRH2 (red). (D) GnRHR2 localizes to both the tubular and interstitial compartments of the porcine testis. Representative immunohistochemistry image (400×) of porcine testes (n = 7) using an antibody directed against GnRHR2 (purple). Reprinted from Desaulniers et al. (35) with permission from Oxford University Press.