Figure 6.

Subcellular localization of the GnRHR2 in a swine testis (ST)-derived cell line. Immunocytochemistry was performed on ST cells (CRL-1746; American Type Culture Collection, Rockville, MD, USA) with an antibody directed against GnRHR2 (1:100–1:200; sc-162889; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and labeled with an Alexa Fluor 488 secondary antibody (green); nuclei were stained with DAPI (blue). The experiment was replicated three times. Two representative confocal microscopy images are shown. Note the plasma membrane and perinuclear staining (arrows). The scale bar represents 10 µm. Adapted from Cederberg et al. (48).