Table 4.

Production of GnRH2 and GnRHR2 in mammalian cell lines.

Cell line	Origin	Species	GnRH2a	GnRHR2a	Reference
Nervous
TE671	Neuronal medulloblastoma	Human	+		(41)
Lung
A549	Alveolar adenocarcinoma	Human		+	(11)
Digestive
SW480	Colorectal adenocarcinoma	Human		+	(11)
IPEC-J2	Intestinal epithelial cells	Porcine		+	(48)
Immune
HL-60	Promyelocytic leukemia	Human		+	(11)
Jukat	T cell leukemia	Human	+	+	(39, 49)
Mammary
MDAMB-231	Breast adenocarcinoma	Human	+		(38)
MCF-7	Breast adenocarcinoma	Human	+		(38)
MCF-10A	Breast epithelium	Human	+		(38)
Female reproductive
HeLa	Cervical adenocarcinoma	Human		+	(11, 50)
Hec-1A	Endometrial adenocarcinoma	Human		+	(51)
Hec-1B	Endometrial adenocarcinoma	Human			(52)
Ishikawa	Endometrial adenocarcinoma	Human		+	(51)
HHUA	Endometrial adenocarcinoma	Human		+	(49)
EFO-21	Ovarian cystadenocarcinoma	Human		+	(51)
EFO-27	Ovarian adenocarcinoma	Human		−	(52)
OVCAR-3	Ovarian adenocarcinoma	Human	+	+	(40, 51)
SK-OV-3	Ovarian adenocarcinoma	Human	+	+	(40, 51)
CaOV-3	Ovarian adenocarcinoma	Human	+		(40)
BG-1	Ovarian adenocarcinoma	Human	+	+	(47, 52)
IOSE-29	Ovarian surface epithelia	Human	+		(40)
SVOG-4O	Granulosa-luteal cells	Human	+		(41)
SVOG-4m	Granulosa-luteal cells	Human	+		(41)
Male reproductive
ST	Fetal testis	Porcine		+	(53)
ALVA-41	Prostate adenocarcinoma	Human		+	(50)
PPC-1	Prostate adenocarcinoma	Human		+	(50)
DU-145	Prostate carcinoma	Human		+	(49, 50)
Urinary
TSU-Pr1	Bladder carcinoma	Human		+	(49, 50)
COS-1b	Kidney	African green monkey		+	(11)
HEK293	Embryonic kidney	Human		+	(50)

^aThe presence of a (+) indicates that either mRNA or protein has been identified, whereas a (−) specifies that the tissue was negative. Blanks designate cell lines that have not yet been examined.

^bThe presence of GnRHR2 protein is putative as GnRH2 treatment of COS-1 cells yielded IP accumulation.