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. 2017 Dec 15;7:17630. doi: 10.1038/s41598-017-17967-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) TEM image of Lipofectamine 2000/pDNA-EGF complexes. (b,c) SEM images of Lipofectamine 2000/pDNA-EGF complexes-loaded collagen-chitosan scaffold (GAS) with different magnifications. The inset image in (c) shows the enlarged view of the highlighted area. Black arrows indicate the Lipofectamine 2000/pDNA-EGF complexes. (d,e) SEM images of GAS/BM-MSCs construct with different magnifications. Green arrows represent the BM-MSCs cultured on GAS. (f) Confocal laser scanning microscopy image of GAS/BM-MSCs construct after being cultured for 7 days, in which nucleus was stained with DAPI (Blue), and cytoskeleton was stained with rhodamine phalloidine (Red).