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. 2017 Dec 15;7:17665. doi: 10.1038/s41598-017-17751-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of ten differential expressed genes in lipid conditions by qRT-PCR. (A) Absolute gene expression during DE (gray bars), LE (black bars), DS (light blue bars), LS (dark blue bars), DNRP1 (light green bars) and LNRP1 (dark green bars) conditions. Data were normalized to the number of copies of rrs gene and results are expressed as log₁₀ of the mean ± SD number of copies per microgram of RNA. Asterisks indicate significant diference between a given lipid stage compared to its respective dextrose stage (LE vs DE, LS vs DS and LNRP1 vs DNRP1), with P < 0.05 considered statistically significant. (B) The relative quantification is expressed as the ratio of [transcription in LE/transcripion in DE] (black bars), the ratio of [transcription in LS/transcription in DS] (blue bars) and the ratio of [transcription in LNRP1/transcription in DNRP1] (green bars).