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. 2017 Dec 15;7:17639. doi: 10.1038/s41598-017-17918-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) D_toStart is constrained to a narrow range in all B. subtilis putative SD sequences, but the optimal range varies depending on the terminus of the 3′ TAIL. (b) Difference in motif length preference of SD sequences with D_toStart = 17 in HEGs and LEGs.