Table 1.

The CRISPR targeting sequences design based on the Optimized CRISPR Design web tool.

AMPKα1	Sequence	Score	Targeted exon
Guide #1	5′-CACCGAAGATCGGCCACTACATTC-3′	90%	1
Guide #2	5′-CACCGATTCGGAGCCTTGATGTGGT-3′	80%	2
Guide #3	5′-CACCGCAGATGGTGTACTGATGACC-3′	74%	3
AMPKα2	Sequence	Score	Targeted exon
Guide #1	5′-CACCGAAGATCGGACACTACGTGC-3′	94%	1
Guide #2	5′-CACCGCTGGGCGACACGCTGG GCGT-3′
	84%	1
Guide #3	5′- CACCGATTCGCAGTTTAGATGTTGT -3′	76%	2
AMPKα1 + α2	Sequence	Score	Targeted exon
Guide #1	5′-CACCGAAGATCGGCCACTACATTC-3′	90%	Exon 1 for AMPKα1 gene
Guide #2	5′-CACCGATTCGGAGCCTTGATGTGGT-3′	80%	Exon 2 for AMPKα1 gene
Guide #3	5′-CACCGAAGATCGGACACTACGTGC-3′	94%	Exon 1 for AMPKα2 gene
Guide #4	5′-CACCGCTGGGCGACACGCTGGGCGT-3′	84%	Exon 1 for AMPKα2 gene