Figure 2.

Macrophages Are Responsible for Increased Metastasis in Cav1 KO→WT Chimeras

(A) Cav1 expression in CD45⁺CD11b⁻ non-myeloid cells, CD45⁺CD11b⁺F4/80⁺ macrophages (TAMs), CD45⁺CD11b⁺Ly6G⁺ neutrophils, and other CD45⁺CD11b⁺Ly6G⁻F4/80⁻ myeloid cells derived from the primary tumor and CD45⁺CD11b⁻ non-myeloid cells, CD45⁺CD11b⁺F4/80⁺ macrophages (MAMs), CD45⁺CD11b⁺Ly6G⁺ neutrophils, and other CD45⁺CD11b⁺Ly6G⁻F4/80⁻ myeloid cells sorted from metastatic nodules of LLC-tumor-bearing mice. n = 4 mice.

(B–D) Tumor growth (B), weight (C), and number of lung metastatic nodules (D) in LLC-tumor-bearing mice treated with macrophage-depleting clodronate liposomes or PBS. n = 11 mice per genotype.

(E and F) F4/80⁺ macrophage accumulation in LLC metastasis (E) and representative images (F). Scale bar, 100 μm. n = 8 mice per genotype.

^§p < 0.05 versus other cell populations, ^∗p < 0.05 versus WT→WT, and ^#p < 0.05 versus PBS. ns, not significant. All graphs show mean ± SEM.